Generally, BIRD is not the preferred fragmentation technique if only MS/MS of peptides and proteins for protein identification is required. Due to these trapping requirements, BIRD is typically only performed in FT-ICR instruments. For peptides and proteins, a BIRD spectrum can take between 10 and 1,000 s to obtain. In addition, because BIRD is a relatively slow process, trapped ions must be stored for longer periods of time in order to obtain sufficient fragmentation. One requirement is that the ambient pressure is relatively low (below 10 −6 Torr), such that photon absorption and emission significantly competes with collisional activation. There are two essential requirements for the successful implementation of BIRD. The absorption of thermal, infrared radiation increases the temperature of ions trapped in the ICR cell, resulting in subsequent fragmentation. Such heating results in the emission of blackbody infrared photons from the walls of the analyzer cell, which are absorbed by trapped ions. Adamson, Kristina Hakansson, in Lectins, 2007 6.4 Blackbody infrared radiative dissociationīlackbody infrared radiative dissociation is achieved in FT-ICR MS by heating the vacuum chamber surrounding the analyzer cell. Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Lectin Analysis
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